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Updated: Sep 27, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
[The effects of quercetin and isorhamnetin on oxidative modification of VLDL induced by Cu2+]
1Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Objective:
To observe the effects of quercetin(Que) and isorhamnetin (Iso) on the oxidative modification of very low density lipoprotein (VLDL).
Methods:
Human VLDL was prepared by one-step ultracentrifuge and oxidized by Cu2+ in vitro; Que and Iso in different concentrations were added to VLDL before incubation with Cu2+. The MDA, vitamin E contents and the activity of superoxide dismutase(SOD) in ox-VLDL were determined.
Results:
Compared with ox-VLDL group, Que and Iso decreased MDA production in a dependent concentration-manner, Que and Iso also elevated Vit E level and SOD activity markedly.
Conclusion:
This experiment indicates that Que and Iso have similar inhibitory effects on the oxidative modification of VLDL.
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