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STRP screening sets for the human genome at 5 cM density
Nader Ghebranious1, David Vaske, Adong Yu
1Molecular Diagnostic Genotyping Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA. ghebranious.nader@marshfieldclinic.org
BMC Genomics
|February 26, 2003
Summary
New human Short Tandem Repeat Polymorphisms (STRPs) screening sets enhance gene mapping. These sets offer improved STRP density and cost-effectiveness for genetic studies.
Area of Science:
- Genetics
- Genomics
Background:
- Short tandem repeat polymorphisms (STRPs) are essential for gene mapping.
- Higher STRP density is crucial for complex genetic disorders and association studies.
Purpose of the Study:
- To develop new human STRP screening sets for enhanced gene mapping.
- To improve the quality and cost-effectiveness of genotyping.
Main Methods:
- Development of two human STRP Screening Sets (Sets 12 and 52).
- Combination of sets to achieve comprehensive genome coverage.
- Utilized tri- and tetranucleotide repeat STRPs.
- Corrected primer sequences for existing STRPs.
Main Results:
- Two new STRP Screening Sets (Sets 12 and 52) developed, spanning all human chromosomes.
- Combined sets contain 782 STRPs with average spacing of 4.8 cM.
- Average heterozygosity of 0.72 and total sex-average coverage of 3535 cM.
- Sets primarily use tri- and tetranucleotide repeats.
Conclusions:
- The new STRP Screening Sets enhance the quality of genotyping.
- These sets improve the cost-effectiveness of gene mapping and association studies.
- Updated information available on the Marshfield Clinic Genetics website.