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Updated: Aug 18, 2026

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
[Influence of different DNA extractions on the identification of streptococcus sanguis group by arbitrary primed
Wei-Dong Zhang1, Hui Chen, Zhong-Sheng Yu
1The Affiliated Hospital of Stomatology, College of Medical Sciences, Zhejiang University, Hangzhou 310031, China.
Abstract:
OBJECTIVE: To assess the influence of different DNA extractions on the identification of streptococcus sanguis group (SSG) species by arbitrary primed polymerase chain reaction (AP-PCR). METHODS: AP-PCR was used to distinguish SSG species by designing 25bp arbitrary primer 5'AAG AGA GGA GCT AGC TCT TCT TGG A 3', and the genomic DNA was extracted by 3 methods. RESULTS: There were great differences in the main band of DNA polymorphism among SSG species. The similar band could be got from the different DNA extractions in the same species. CONCLUSION: Different DNA extractions have no influence on the identification of SSG.
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