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Updated: Aug 18, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
Prediction of boundaries between intrinsically ordered and disordered protein regions
Predrag Radivojac1, Zoran Obradović, Celeste J Brown
1Center for Information Science and Technology, Temple University, USA.
Abstract:
Using proteins with both disordered and ordered regions collected through literature searches and database scanning, we assembled a set of 24-residue long segments centered on their order/disorder boundaries as well as a larger set of non-boundary segments consisting of either order or disorder. We analyzed position-specific amino acid compositions around the order/disorder boundaries and found more than thirty significant (p < 0.05) compositional differences between boundary and non-boundary data. From this analysis, we constructed several logistic regression predictors of order/disorder boundaries using slightly different data modeling approaches. Exact boundary prediction accuracies were estimated to be in the range from 74% to 80% for the different predictors.
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