Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A conformationally preorganized universal solid support for efficient oligonucleotide synthesis.

Andrei P Guzaev1, Muthiah Manoharan

  • 1Department of Medicinal Chemistry, Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA. aguzaev@isisph.com

Journal of the American Chemical Society
|February 27, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and biophysical evaluation of C-5-triazolyl-functionalized morpholino-thymidine analogs.

Organic & biomolecular chemistry·2026
Same author

Acyclic serinol nucleic acid modification of siRNAs overcomes seed region mediated off-target effects while maintaining potency.

Nucleic acids research·2026
Same author

Synthesis of siRNAs containing carbocyclic nucleotides and the role of cyclopentane conformation in RNAi activity.

RSC chemical biology·2026
Same author

C‑Nucleosides Stabilize RNA by Reducing Nucleophilicity at 2'-OH.

ACS central science·2025
Same author

Synthesis and Biophysical Properties of 3'-Deoxy-β-d-apio-d-furanosyl Nucleic Acids.

ACS chemical biology·2025
Same author

Modified unlocked nucleic acid (MUNA) mitigates off-target effects of small interfering RNAs.

Nucleic acids research·2025
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

A new solid support simplifies oligonucleotide synthesis by enabling easy release of synthesized DNA or RNA strands. This method is effective for various oligonucleotide types, including phosphorothioate analogues.

Area of Science:

  • Organic Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Oligonucleotide synthesis is crucial for molecular biology and therapeutics.
  • Current solid support methods can be inefficient or require harsh conditions for product release.

Purpose of the Study:

  • To develop a novel, conformationally preorganized nonnucleosidic universal solid support for efficient oligonucleotide synthesis.
  • To optimize the release conditions for synthesized oligonucleotides from the solid support.

Main Methods:

  • Design and synthesis of a novel solid support with orthogonally protected hydroxyl groups.
  • Extensive testing in the preparation of various oligonucleotides and phosphorothioate analogues.
  • Kinetic studies of the oligonucleotide release process using concentrated ammonium hydroxide.

Related Experiment Videos

Main Results:

  • The novel solid support facilitates the preparation of diverse oligonucleotides, including those with modified nucleosides.
  • Oligonucleotides are efficiently released from the support via transesterification upon deprotection.
  • Optimal release conditions were determined based on oligonucleotide backbone and 3'-terminal nucleoside chemistry.

Conclusions:

  • The developed solid support offers a facile and efficient method for oligonucleotide synthesis and release.
  • This advancement provides a versatile tool for preparing oligonucleotides for research and therapeutic applications.
  • The study elucidates optimal conditions for releasing various oligonucleotide types, enhancing synthetic utility.