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Related Experiment Videos

Visualization of oleic acid-induced transdermal diffusion pathways using two-photon fluorescence microscopy.

Betty Yu1, Ki Hean Kim, Peter T C So

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A.

The Journal of Investigative Dermatology
|February 27, 2003
PubMed
Summary
This summary is machine-generated.

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Dual-channel two-photon fluorescence microscopy reveals new skin penetration pathways. Intracorneocyte diffusion occurs for both hydrophobic and hydrophilic drugs, independent of oleic acid enhancers.

Area of Science:

  • Dermatology
  • Biophysics
  • Pharmacology

Background:

  • Transdermal drug delivery relies on understanding skin barrier function.
  • Current models primarily focus on lipid multilamellar pathways for drug permeation.
  • The role of intracellular diffusion within corneocytes is less understood.

Purpose of the Study:

  • To investigate simultaneous skin autofluorescence and transdermal model drug distribution.
  • To explore novel skin penetration pathways beyond the lipid route.
  • To analyze the impact of oleic acid on drug diffusion based on physicochemical properties.

Main Methods:

  • Utilized dual-channel high-speed two-photon fluorescence microscopy.
  • Simultaneously imaged endogenous skin fluorophores and rhodamine-based model drugs.

Related Experiment Videos

  • Quantified spatial distributions of model drugs across skin structural features.

    • Main Results:

    • Provided evidence for intracorneocyte diffusion alongside the lipid multilamellar pathway.
    • Demonstrated intracorneocyte diffusion for both hydrophobic and hydrophilic model drugs without oleic acid.
    • Showed oleic acid's mechanism depends on drug properties: localization for hydrophobic, partitioning for hydrophilic drugs.

    Conclusions:

    • Intracorneocyte diffusion is a significant, previously underappreciated, transdermal pathway.
    • Model drug properties dictate interactions with the skin barrier and enhancers like oleic acid.
    • Dual-channel microscopy offers a powerful tool for dissecting complex skin permeation mechanisms.