Simple fluorescent PCR method for detection of large deletions in the beta-globin gene cluster
Tiago Gomes De Andrade1, Sara Teresinha Olalla Saad, Maria de Fatima Sonati
1Hematology-Hemotherapy Center, State University of Campinas, UNICAMP, Brazil.
Abstract:
We developed a semi-automated approach to detect large deletions in the beta-globin gene cluster, based on the quantitative differences in the amplifications of samples by a fluorescent PCR. With this strategy, we were able to detect the presence of HPFH-2 in an African-Brazilian subject, confirmed by sequencing analysis. Differently from other PCR strategies, GAP-PCR for example, it has the potential to identify new deletions.
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