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Related Experiment Videos

Nitrogen fixation associated with grasses in Oregon.

A D Nelson, L E Barber, J Tjepkema

    Canadian Journal of Microbiology
    |April 1, 1976
    PubMed
    Summary

    This study investigated nitrogen fixation in Oregon grasslands and crops. Agrostis tenuis demonstrated significant nitrogen fixation, potentially crucial for its survival, with associated nitrogen-fixing microbes identified.

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    Area of Science:

    • Agricultural Science
    • Microbiology
    • Ecology

    Background:

    • Nitrogen fixation is a vital process for plant growth and ecosystem health.
    • Understanding nitrogen fixation in diverse plant communities is crucial for sustainable agriculture and natural resource management.

    Purpose of the Study:

    • To quantify nitrogen fixation rates in Oregon's natural grasslands and grain crops.
    • To identify nitrogen-fixing microorganisms associated with grass species.

    Main Methods:

    • Acetylene-reduction assay was employed to measure nitrogenase activity.
    • Microorganisms were isolated from grass root surfaces for identification.

    Main Results:

    • Agrostis tenuis exhibited the highest acetylene-reducing activity, estimated at 37 g N2 fixed per hectare per day.

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  • An estimated 3 kg of N2 could be fixed per hectare annually by Agrostis tenuis.
  • Nitrogen-fixing bacteria, including Bacillus macerans, Bacillus polymyxa, and Enterobacter cloacoa, were isolated from wheat and other grasses.

    • Conclusions:

    • Nitrogen fixation by Agrostis tenuis is substantial and likely plays a key role in its ecological persistence.
    • The identified nitrogen-fixing bacteria contribute to the nitrogen cycle in grassland and agricultural ecosystems.