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Published on: February 13, 2019
Competitive interactions of adriamycin and ethidium bromide with DNA as studied by full rank parallel factor analysis
Hong-Ping Xie1, Xia Chu, Jian-Hui Jiang
1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemometrics and Chemical Engineering, Institute for Chemometrics and Chemical Technology, Hunan University, Changsha 410082, People's Republic of China.
Abstract:
The competitive interactions of adriamycin (AMC) and a fluorescence probe of ethidium bromide (EB) with DNA have been studied by full rank parallel factor analysis (FRA-PARAFAC) of fluorescence excitation-emission three-way data array. The excitation and emission spectra as well as the equilibrium concentrations of co-existing species in different reaction mixtures can be directly obtained by the FRA-PARAFAC treatment. The concordance of the resolved excitation and emission spectra of AMC, EB and EB-DNA with the standard spectra of these species confirmed the reliability of the equilibrium concentrations of these components in the reaction mixtures studied. The results obtained are valuable for providing a deeper insight into the competitive interaction mechanism of AMC and EB with DNA. The conclusion was directly given out that the interaction of AMC with DNA is the intercalating model. The FRA-PARAFAC method as exemplified by the present study provides an useful approach for studying the interaction of clinical drugs with DNA in the presence of disturbance of drug assistants.
