Ammonium hydrogencarbonate, an excellent buffer for the analysis of basic drugs by liquid chromatography-mass
Alfonso Espada1, Alfonso Rivera-Sagredo
1DCR&T Alcobendas, European Analytical Technologies, Lilly S.A., Avda. de la Industria 30, 28108 Alcobendas, Spain.
Abstract:
Ammonium hydrogencarbonate buffer has been found to be especially useful for high-pH HPLC analysis of samples from both combinatorial and medicinal chemistry sources. Satisfactory results were obtained by the standard diode array, evaporative light-scattering, and MS detection by using this buffer at a concentration of 10 mM. From a practical standpoint, ammonium hydrogencarbonate is an ideal buffer for chromatographers since it provides excellent chromatographic behaviour and reproducible separation. In addition to this, its volatility makes it an essential tool for rapid LC-MS product identification. Ammonium hydrogencarbonate was tested for a number of drug-like compounds analysed as mixtures, and data obtained were compared to those from the classical and MS-friendly buffers widely used by chromatographers: trifluoroacetic and formic acids. The results of this study revealed the suitability of this buffer for routine HPLC application in research laboratories.
More Related Videos
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Related Concept Videos
Titration of a Weak Base with a Strong Acid
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...
Buffers
Phosphate Buffer
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
Ion-Exchange Chromatography
Bicarbonate-Carbonic Acid Buffer
Titration of Polyprotic Base with a Strong Acid
