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Updated: Aug 17, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Two forms of RNA polymerase II holoenzyme display different abundance during the cell cycle
Linda Holland1, Krassimir Yankulov
1Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario, Canada N1G 2W1. lholland@uoguelph.ca
Abstract:
We analyzed the composition and abundance of two forms of RNA polymerase II (pol II) holoenzyme in synchronized HeLa cells. We did not detect significant changes in pol II holoenzyme composition, but we noticed differences in the abundance of the two complexes at different stages of the cell cycle. Summarized data from several independent experiments demonstrate that pol II holoenzyme, which is purified by GST-TFIIS affinity chromatography, is more abundant during G1/S and S phases. Another form of pol II holoenzyme, which is purified by anti-CDK7 antibodies, shows relatively higher amounts in G2/M and early G1 phases.
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