Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Macroporous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) matrices for bone tissue engineering.

G Torun Köse1, H Kenar, N Hasirci

  • 1Biotechnology Research Unit, Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey.

Biomaterials
|March 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach.

Journal of endocrinological investigation·2023
Same author

Anatomical meniscus construct with zone specific biochemical composition and structural organization.

Biomaterials·2019
Same author

Square prism micropillars improve osteogenicity of poly(methyl methacrylate) surfaces.

Journal of materials science. Materials in medicine·2018
Same author

Effects of microarchitecture and mechanical properties of 3D microporous PLLA-PLGA scaffolds on fibrochondrocyte and L929 fibroblast behavior.

Biomedical materials (Bristol, England)·2018
Same author

EFFECTS OF ETHYLENE OXIDE RESTERILISATION AND IN-VITRO DEGRADATION ON MECHANICAL PROPERTIES OF PARTIALLY ABSORBABLE COMPOSITE HERNIA MESHES.

East African medical journal·2016
Same author

Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells.

Journal of tissue engineering and regenerative medicine·2015

This study developed porous poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) scaffolds for bone regeneration. The macroporous PHBV matrices supported osteoblast growth and proliferation, indicating their potential for tissue engineering applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Macroporous scaffolds are crucial for bone tissue regeneration.
  • Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) is a biodegradable polymer with potential for biomedical applications.
  • Surface modification of scaffolds can enhance cell attachment and proliferation.

Purpose of the Study:

  • To prepare macroporous PHBV matrices with controlled porosity.
  • To investigate the effect of rf-oxygen plasma treatment on PHBV surface properties.
  • To evaluate the suitability of these PHBV scaffolds for osteoblast attachment and proliferation.

Main Methods:

  • Macroporous PHBV matrices were fabricated using solvent evaporation and solute leaching with sucrose crystals.

Related Experiment Videos

  • Scanning electron microscopy (SEM) and image analysis were used to characterize surface morphology and pore size distribution.
  • rf-oxygen plasma treatment was applied to modify the PHBV surface.
  • Osteoblast proliferation on the scaffolds was assessed using SEM after in vitro incubation.

    • Main Results:

    • Foam preparation conditions significantly influenced void volume, pore size, and density.
    • rf-oxygen plasma treatment altered surface chemistry and hydrophilicity.
    • PHBV foams exhibited good stability in aqueous media for 120 days.
    • Osteoblast growth and proliferation were observed on the PHBV matrices after 29 and 60 days of incubation.

    Conclusions:

    • Macroporous PHBV matrices can be successfully prepared with tunable properties.
    • Surface modification enhances the potential of PHBV scaffolds for osteoblast seeding.
    • These PHBV scaffolds demonstrate promise as a biomaterial for bone tissue engineering applications.