Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnetic order and competition with superconductivity in (Ho-Er)Ni<sub>2</sub>B<sub>2</sub>C.

Materials research express·2023
Same author

Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach.

Journal of endocrinological investigation·2023
Same author

Anatomical meniscus construct with zone specific biochemical composition and structural organization.

Biomaterials·2019
Same author

Square prism micropillars improve osteogenicity of poly(methyl methacrylate) surfaces.

Journal of materials science. Materials in medicine·2018
Same author

Effects of microarchitecture and mechanical properties of 3D microporous PLLA-PLGA scaffolds on fibrochondrocyte and L929 fibroblast behavior.

Biomedical materials (Bristol, England)·2018
Same author

Pain intensity and its objective determinants following implant surgery and sinus lifting: A 1-year prospective study.

Nigerian journal of clinical practice·2017

Related Experiment Video

Updated: Apr 19, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K

Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells.

A C Calikoglu Koyuncu1, G Gurel Pekozer1, M Ramazanoglu2

  • 1Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey.

Journal of Tissue Engineering and Regenerative Medicine
|January 6, 2015
PubMed
Summary
This summary is machine-generated.

Human tooth germ stem cells (HTGSCs) differentiated into chondrocytes on 3D scaffolds, showing potential for cartilage regeneration. The PCL-PLLA blend scaffold demonstrated superior results for cell differentiation and matrix deposition.

Keywords:
cartilage tissue engineeringcell differentiationhuman tooth germ stem cellspoly(l-lactide)polycaprolactonepolyester scaffolds

More Related Videos

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

3.0K
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

20.8K

Related Experiment Videos

Last Updated: Apr 19, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K
Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering
09:49

Author Spotlight: Insights into the Use of Apple-Derived Cellulose Scaffolds for Bone Tissue Engineering

Published on: February 23, 2024

3.0K
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
12:37

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

Published on: October 7, 2015

20.8K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Cartilage defects pose significant clinical challenges.
  • Stem cells offer a promising source for cartilage regeneration.
  • Biomaterial scaffolds are crucial for guiding cell differentiation and tissue formation.

Purpose of the Study:

  • To investigate the chondrogenic differentiation of human tooth germ stem cells (HTGSCs) on three-dimensional (3D) scaffolds.
  • To evaluate the suitability of polycaprolactone (PCL), poly(L-lactic acid) (PLLA), and a PCL-PLLA blend scaffold for cartilage regeneration.
  • To assess cell proliferation and extracellular matrix (ECM) deposition on different scaffold materials.

Main Methods:

  • Isolation and culture of HTGSCs from impacted third molar tooth germs.
  • Seeding of HTGSCs onto PCL, PLLA, and PCL-PLLA scaffolds.
  • Culture in chondrogenic differentiation medium for 3 weeks.
  • Assessment of cell proliferation using MTS assay.
  • Immunohistochemical analysis for cell attachment and ECM deposition.
  • Real-time PCR to quantify cartilage-specific gene expression (Collagen Type II, Aggrecan).

Main Results:

  • All tested scaffolds (PCL, PLLA, PCL-PLLA) supported HTGSC proliferation.
  • HTGSCs successfully attached to the scaffolds and deposited cartilage-specific ECM.
  • The PCL-PLLA blend scaffold showed the highest expression of Collagen Type II and Aggrecan after 3 weeks.
  • Scaffold topographies revealed interconnected macroporous structures suitable for cell infiltration.

Conclusions:

  • All evaluated 3D scaffolds are suitable for chondrogenic differentiation of HTGSCs.
  • The PCL-PLLA blend scaffold demonstrates enhanced potential for cartilage regeneration applications.
  • HTGSCs represent a viable cell source for developing cartilage repair strategies using biomaterial scaffolds.