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Related Experiment Videos

Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain.

Shigemi Hayashi1, Paula Lewis, Larysa Pevny

  • 1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.

Gene Expression Patterns : GEP
|March 6, 2003
PubMed
Summary

A new transgenic mouse line, Sox2Cre, enables precise gene manipulation in embryonic epiblast cells. This tool facilitates the study of developmental genes like Sonic hedgehog, offering improved efficiency over existing methods for gene knockout studies.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The Sox2 gene plays a crucial role in early embryonic development.
  • Precise genetic tools are needed to study gene function specifically in the epiblast.

Purpose of the Study:

  • To generate and characterize a novel transgenic mouse line (Sox2Cre) for epiblast-specific gene manipulation.
  • To compare the efficiency of Sox2Cre with existing Cre driver lines.

Main Methods:

  • Generation of a Sox2Cre transgenic mouse line.
  • Utilizing a R26R reporter line to assess Cre activity.
  • Crossing Sox2Cre mice with conditional Sonic hedgehog (Shh) alleles.
  • Comparison with the Mox2Cre (MORE) transgenic line.

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Main Results:

  • Sox2Cre drives recombination in all epiblast cells by embryonic day 6.5.
  • Sox2Cre-Cre-mediated recombination in Shh conditional mutants phenocopies Shh null mutants.
  • Sox2Cre shows higher efficiency and earlier activity than MORE in epiblast recombination.

Conclusions:

  • The Sox2Cre line is a powerful tool for achieving complete, epiblast-specific gene inactivation.
  • Sox2Cre offers advantages over MORE for studying essential genes in early development.
  • The MORE line's mosaic activity is useful for generating partial loss-of-function phenotypes.