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Two-Photon In Vivo Imaging of the Mouse Retina

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Source: Wang, Z., et al. Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina. J. Vis. Exp. (2021).This video demonstrates a technique for in vivo retinal imaging using two-photon microscopy. Anesthetized mice are prepared for imaging by securing their heads, Applying eye lubricant, and placing a coverslip over the eye. Retinal ganglion cells (RGCs) expressing fluorescent proteins are first visualized using epifluorescence for alignment. Finally, two-photon imaging is...

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Related Experiment Video

Updated: Jan 20, 2026

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Published on: September 5, 2019

Photonics for biologists

¹Department of Neurobiology and Behavior, University of California, Irvine 92697, USA.

Methods in Enzymology

|March 8, 2003

Summary

No abstract available in PubMed .

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