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Hematologic and serum biochemical values for zebrafish (Danio rerio)
Jill M Murtha1, Weici Qi, Evan T Keller
1Unit for Laboratory Animal Medicine, Department of Pathology, University of Michigan, Room 5304 CCGCB, 1500 E. Medical Center Drive, Ann Arbor, Michigan 48109-0940, USA.
Comparative Medicine
|March 11, 2003
Summary
This study establishes baseline hematologic and biochemical parameters for adult zebrafish (Danio rerio), identifying predominantly lymphocytes and normal red blood cell counts. These findings are crucial for aging and disease research using zebrafish models.
Area of Science:
- Comparative physiology
- Aquatic animal models
- Vertebrate genetics
Background:
- Zebrafish (Danio rerio) are widely used in developmental and genetic studies.
- Most research focuses on embryonic stages, leaving adult characteristics underexplored.
- Adult zebrafish hold potential for studying aging and age-related diseases.
Purpose of the Study:
- To characterize basic hematologic and biochemical parameters in mature zebrafish.
- To establish reference values for adult zebrafish blood.
- To support the use of zebrafish as a model for aging and disease research.
Main Methods:
- Blood collection from adult zebrafish.
- Analysis of hematologic parameters, including white blood cell differentials and red blood cell counts.
- Serum biochemical analysis of various analytes.
Main Results:
- White blood cell differential counts showed a predominance of lymphocytes (mean 82.95%).
- Total red blood cell counts averaged 3.02 x 10^6 cells/microliter.
- Most serum biochemical analytes were within normal ranges, with exceptions in alanine transaminase (ALT), amylase, and phosphorus.
Conclusions:
- Established reference values for adult zebrafish blood parameters.
- Highlights the potential of adult zebrafish for aging and disease research.
- Provides essential data for accurate analysis of zebrafish mutants in future studies.