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Functional EF-Tu with large C-terminal extensions in an E coli strain with a precise deletion of both chromosomal tuf
Robert Schnell1, Farhad Abdulkarim, Miklós Kálmán
1Department of Microbiology, Stockholm University, S-106 91 Stockholm, Sweden.
FEBS Letters
|March 14, 2003
Abstract:
An Escherichia coli strain was constructed in which both chromosomal genes encoding elongation factor (EF)-Tu (tufA and tufB) have been inactivated with precise coding sequence replacements. A tufA gene in an expression vector is supplied as the sole EF-Tu source. By using plasmid replacement, based on plasmid incompatibility, mutant EF-Tu variants with a large C'-terminal extension up to 270 amino acids were studied and proved to be functional in a strain lacking the chromosomal tufA and tufB genes.