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Updated: Sep 26, 2026
Intracellular Refolding Assay
Published on: January 24, 2012
Clinical significance of heat shock protein-70 expression in bladder cancer
Konstantinos N Syrigos1, Kevin J Harrington, Anastasios J Karayiannakis
1Laboratory of Molecular Oncology, 3rd Department of Internal Medicine, Sotiria General Hospital, Athens University School of Medicine, Athens, Greece.
Objectives:
To investigate the expression of heat shock protein-70 (HSP-70) in transitional cell bladder cancer and correlate it with normal and inflammatory bladder tissue samples.
Methods:
We used a three-step avidin-biotin method to examine the localization of a rabbit anti-HSP-70 polyclonal antibody in normal (n = 10), inflammatory (n = 10), and malignant (n = 67) bladder tissue samples.
Results:
Fifty-eight percent of the bladder cancer samples and only 10% and 20% of the normal and inflammatory bladder tissue samples, respectively, expressed HSP-70. Localization of the antibody correlated with the grade (P <0.001), stage (P <0.001), and survival (P <0.05) of the patients with malignancy.
Conclusions:
HSP-70 is frequently overexpressed by bladder cancer cells and could be used as biochemical marker in patients with bladder cancer.
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