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[Improvement of cardiomyocyte isolation for different purposes]
Chun-Mei Cao1, Xiong Zhang, Ying-Ying Chen
1Department of Physiology, College of Medical Sciences, Zhejiang University Hangzhou 310031, China.
Objective:
To improve the methods of isolating single ventricular myocytes for purposes of different experiments.
Methods:
The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion technique at constant flow rate. The patch clamp whole-cell recording, a spectrofluorometric method and a video tracking system were used to verify the basic electrophysiological properties, intracellular calcium transient and cell contraction of the single myocytes.
Results:
The resting membrance potential of the myocyte was (-74.06+/-4.54)mV. The whole-cell sodium, potassium and calcium currents, intracellular calcium transients and contractile properties were stable and typical.
Conclusion:
The myocytes obtained by adjusting perfusion parameters are well suited for electrophysiological recording, intracellular calcium and single cell contraction measurements.