Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two tcf3 genes cooperate to pattern the zebrafish brain.

Richard I Dorsky1, Motoyuki Itoh, Randall T Moon

  • 1Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA. richard.dorsky@hsc.utah.edu

Development (Cambridge, England)
|March 19, 2003
PubMed
Summary

Two Tcf3 homologs, Headless (Hdl) and Tcf3b, regulate gene expression in the developing nervous system. Tcf3b limits caudalization and plays a unique role in brain development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Age-dependent vascular and neurological characteristics of CADASIL are recapitulated in Notch3 mutant zebrafish, implicating a role for type IV collagen in disease progression.

Acta neuropathologica communications·2026
Same author

Exploring Self-Organization of Development in the Zebrafish Posterior Lateral Line Primordium with Computer Models.

Cold Spring Harbor perspectives in biology·2026
Same author

Non-Mammalian Models in Ischemic Stroke Research: Advances, Applications, and Translational Potential.

Journal of neuroscience research·2026
Same author

High-fat diet impairs intermediate-term memory by autophagic-lysosomal dysfunction in Drosophila.

PLoS genetics·2025
Same author

Aberrant humoral immune responses and intestinal homeostasis in Cd38 Bst1 double knockout mice.

ImmunoHorizons·2025
Same author

Synergistic effects of mutation and glycosylation on disease progression.

Frontiers in molecular biosciences·2025

Area of Science:

  • Developmental biology
  • Neuroscience
  • Genetics

Background:

  • Caudalizing factors and Wnt/beta-catenin signaling pattern the vertebrate nervous system.
  • Headless (Hdl), a Tcf3 homolog, represses caudal genes in zebrafish.
  • Understanding Tcf3 homologs is crucial for deciphering neural development.

Purpose of the Study:

  • Investigate the role of a second Tcf3 homolog, Tcf3b, in neural development.
  • Determine how Tcf3b interacts with Hdl in regulating caudalization.
  • Explore the function of Tcf3b in brain morphogenesis.

Main Methods:

  • Zebrafish genetics to study Tcf3 homologs (Hdl and Tcf3b).
  • Analysis of gene expression changes in response to altered Tcf3 function.
  • Functional rescue experiments using Tcf3 homologs.

Related Experiment Videos

Main Results:

  • Tcf3b limits caudalization caused by loss of Hdl function.
  • Tcf3b can rescue hdl mutants, while Lef1 cannot.
  • Wnts antagonize Tcf3-mediated repression via a beta-catenin binding domain.
  • Reduced Tcf3 function predicts caudalizing activity gradients.
  • Tcf3b has a unique role in rhombomere boundary morphogenesis.

Conclusions:

  • Tcf3b acts as a critical regulator of caudalization and neural patterning.
  • Tcf3b possesses distinct functions in brain development beyond caudal gene regulation.
  • The interplay between Tcf3 homologs and Wnt signaling shapes the developing nervous system.