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Biomarker discovery in urine by proteomics
James X Pang1, Nicole Ginanni, Ashok R Dongre
1Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Princeton, New Jersey 08543, USA.
Journal of Proteome Research
|March 20, 2003
Summary
Researchers successfully identified potential disease biomarkers in urine proteins using advanced proteomics. This study demonstrates urine proteomics can detect inflammation markers like orosomucoid, supporting its use in diagnostics.
Area of Science:
- Proteomics
- Biomarker Discovery
- Clinical Chemistry
Background:
- Identifying disease biomarkers is crucial for early diagnosis and treatment.
- Urine proteomics offers a non-invasive approach for biomarker discovery.
- Previous studies suggest urine protein profiles can change with disease states.
Purpose of the Study:
- To test the hypothesis that adequate protein can be isolated from urine for biomarker identification.
- To identify potential urinary biomarkers for an acute inflammation state using proteomics.
- To evaluate the utility of different proteomics techniques for analyzing urine proteins.
Main Methods:
- Urine samples were collected from a healthy volunteer during acute inflammation and recovery.
- Proteins and peptides were concentrated and desalted using solid-phase extraction.
- Proteomic analysis was performed using 2D gel electrophoresis and liquid chromatography-tandem mass spectrometry.
Main Results:
- A set of proteins was identified as putative biomarkers across all three experimental approaches.
- Identified proteins were previously linked to inflammation in scientific literature.
- Orosomucoid, an acute phase reactant, showed a significant increase in abundance during inflammation.
Conclusions:
- The study supports the hypothesis that urine proteomics can identify disease-specific protein biomarkers.
- Urinary orosomucoid levels can serve as a potential biomarker for acute inflammation.
- Proteomics techniques are effective for analyzing complex protein mixtures in urine.