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Shipping Conditions May Impact the Reliability of At-Home Microsampling Devices.

Andrea Villanueva Raisman1, Jakob Naess1, David Kotol2

  • 1SciLifeLab, Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 171 65 Solna, Sweden.

Journal of Proteome Research
|July 14, 2026
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Summary

Dried blood spot (DBS) microsampling combined with targeted proteomics shows promise for decentralized healthcare. However, shipping and storage conditions, especially high temperatures, can impact protein quantification reliability.

Keywords:
DBSmass spectrometrymicrosampling

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Targeted proteomics enables precise protein quantification in biological samples, crucial for biomarker discovery and diagnostics.
  • At-home microsampling, particularly using dried blood spots (DBSs), offers potential for population screening and decentralized healthcare.
  • Volumetric microfluidic devices ensure consistent DBS microsampling, but data reliability under various conditions requires validation.

Purpose of the Study:

  • To investigate the impact of shipping conditions and storage on the reliability of protein quantification from DBS microsamples.
  • To assess the stability of biomarkers collected via DBS microsampling under simulated environmental stresses.

Main Methods:

  • Collected 72 deidentified blood samples using the CapitainerB DBS microsampling device.
  • Analyzed samples using targeted mass spectrometry and quantitative recombinant protein standards (qRePSs).
  • Subjected DBS samples to three simulated shipping conditions and two storage container types to evaluate stability.

Main Results:

  • Shipping conditions and high-temperature storage significantly affected protein identification and quantification.
  • Variability in results was observed, indicating a potential impact on biomarker reliability.

Conclusions:

  • Environmental factors during shipping and storage can compromise the integrity of protein data obtained from DBS microsamples.
  • Further research is necessary to establish robust protocols ensuring biomarker reliability in decentralized microsampling settings.