Related Experiment Videos
Synthesis and characterization of covalently linked single-stranded DNA oligonucleotide-dendron conjugates
Stephen A Bell1, Megan E McLean, Sang-Keun Oh
1Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station 77842-3012, USA.
Bioconjugate Chemistry
|March 20, 2003
Abstract:
A solution-phase synthesis and characterization of covalent DNA-dendron conjugates is presented. Thiol-terminated 12-base oligonucleotides were added to second- and third-generation triazine-based dendrons via thiol/disulfide exchange chemistry. Single-stranded DNA oligonucleotides were successfully attached to dendrons at the core, the periphery, and both. Proof of structure for these architectures is derived primarily from mass spectrometry and polyacrylamide gel electrophoresis and complemented by labeling analysis using Ellman's reagent and degradation analysis using a reducing agent.