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Rapid analysis of tryptically digested cerebrospinal fluid using capillary electrophoresis-electrospray
Magnus Wetterhall1, Magnus Palmblad, Per Håkansson
1Institute of Chemistry, Department of Analytical Chemistry, Uppsala University, PO Box 531, SE-751 21 Uppsala, Sweden.
Abstract:
Mass spectrometry has in recent years been established as the method of choice for protein identification and characterization in proteomics. Capillary electrophoresis (CE) is a fast and efficient method for the separation of peptides and proteins. The on-line combination of CE with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) has been shown to be a powerful tool in the analysis of complex mixtures of proteins. This paper presents the first results from a proteomic analysis of human cerebrospinal fluid proteins by tryptic digestion and CE-FTICR-MS, where 30 proteins could be identified on a 95% confidence level with mass measurement errors less than 5 ppm.