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Updated: Sep 26, 2026

Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
Published on: February 27, 2018
Huntingtin-interacting protein-1-related protein of rat (rHIP1R) is localized in the postsynaptic regions
Akira Okano1, Nobuteru Usuda, Kenichi Furihata
1Department of Neuroplasticity, Research Center on Aging and Adaptation, University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
Abstract:
We cloned a rHIP1R (GenBank Accession No., AB005052) encoding a Sla2/huntingtin-interacting protein (HIP1) family protein from a rat brain cDNA library. Localization of rHIP1R was investigated in the rat brain using an antibody specific to the HIP1R antibody. The rHIP1R protein was enriched in the synaptic plasma membrane fraction along with huntingtin, a synaptic protein and a causal protein for Huntington's disease. The electron microscopic examination revealed that HIP1R was localized at postsynaptic spines. Localization of HIP1R in the small vesicular structures in the spine, possible sites of vesicular transport of synaptic proteins, together with the structure-based analysis, suggested a role of HIP1R for vesicle trafficking through interaction with F-actin and working together with huntingtin and HIP1 at the synaptic sites.
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