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Genomics in target and drug discovery
M van Duin1, H Woolson, D Mallinson
1Organon Research Laboratories, Department of Target Discovery, Newhouse, Lanarkshire ML1 5SH, Scotland, U.K. marcel.vanduin@organon.com
Biochemical Society Transactions
|March 26, 2003
Summary
Genomics aids drug target discovery by validating gene expression in controlled experiments. This study demonstrates target identification for atherosclerosis using human macrophage foam cells.
Area of Science:
- Genomics
- Pharmacology
- Cell Biology
Background:
- Genomics-based drug discovery necessitates rigorous experimental design.
- Validating differentially expressed genes is crucial for identifying reliable therapeutic targets.
- Microarray experiments can yield numerous gene candidates requiring further selection.
Purpose of the Study:
- To illustrate a methodology for genomics-based drug target discovery.
- To demonstrate the validation of differentially expressed genes in a specific therapeutic context.
- To identify novel drug targets for atherosclerosis treatment.
Main Methods:
- Designing controlled biological and pharmacological experiments.
- Focusing experimental questions on the therapeutic area of interest.
- Manipulating human macrophage foam cells to study gene expression.
Main Results:
- The presented approach facilitates the validation of differentially expressed genes.
- This method aids in the de-selection of non-relevant genes identified in microarray analyses.
- Novel drug targets for atherosclerosis were identified through this experimental strategy.
Conclusions:
- A systematic approach combining genomics and controlled experiments enhances drug target discovery.
- This methodology is effective for validating gene targets in specific diseases like atherosclerosis.
- The manipulation of human macrophage foam cells provides a viable model for identifying therapeutic targets.