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[A new model of choroidal neovascularization in mice]
Kang Wang1, Kangsun Wang, Ling Wang
1Ophthalmic Center, the Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. lanbowang1972@yahoo.com
Objective:
To investigate the feasibility of inducing choroidal neovascularization (CNV) in mice by diode laser.
Methods:
Two hours, 3 days, 1 week, 2 weeks and 4 weeks after photocoagulation, the effect of diode laser for inducing CNV by fundus fluorescein angiography (FFA), light microscopy and transmission electron microscopy.
Results:
Two hours and 3 days after laser treatment, there were no CNV and fluorescent leakage observed in mice. The first signs of leakage in FFA associated with CNV appeared 1 week after the postphotocoagulation, (48/90 lesions, or 53.3%). Four weeks after laser treatment, there were still 33 lesions appearing leakage (36.67%). Histological sections proved the existence of CNV, but the rate of CNV was not in accord with that of fluorescence leakage.
Conclusions:
This animal model is very useful for in vivo studies of angiogenesis and its modulation via various therapy.