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Related Concept Videos

Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Contractile Ring02:15

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Contractile Ring02:15

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...

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Related Experiment Video

Updated: May 11, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Chromosomal cohesin forms a ring.

Stephan Gruber1, Christian H Haering, Kim Nasmyth

  • 1Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, 1030 Vienna, Austria.

Cell
|March 26, 2003
PubMed
Summary
This summary is machine-generated.

The cohesin complex forms a ring structure essential for sister chromatid cohesion. Its cleavage fragments remain linked, revealing how cohesin detaches from chromosomes during cell division.

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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Capturing Chromosome Conformation Across Length Scales
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Capturing Chromosome Conformation Across Length Scales

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The cohesin complex is crucial for maintaining sister chromatid cohesion during mitosis.
  • Cohesin consists of Smc1, Smc3, and Scc1 subunits, forming a ring-like structure.
  • Separase-mediated cleavage of Scc1 triggers cohesin release at anaphase onset.

Purpose of the Study:

  • To investigate whether cohesin forms a complete ring structure in vivo.
  • To elucidate the mechanism of cohesin release from chromosomes during anaphase.

Main Methods:

  • Analysis of cohesin subunit interactions after Scc1 cleavage.
  • Investigating the role of Smc3 coiled coil cleavage in cohesin release.

Main Results:

  • Scc1 cleavage fragments remain associated with the Smc1/Smc3 heterodimer.
  • Cleavage of the Smc3 coiled coil alone is sufficient to release cohesin from chromosomes.
  • Loss of sister chromatid cohesion correlates with cohesin release.

Conclusions:

  • Cohesin's ring structure is maintained by interactions between Scc1 fragments and the Smc1/Smc3 heterodimer.
  • Topological association with chromatin, mediated by the Smc3 coiled coil, is critical for cohesin's function.