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Scp160p associates with specific mRNAs in yeast
Ai-Min Li1, Alice Watson, Judith L Fridovich-Keil
1Department of Human Genetics, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA.
Nucleic Acids Research
|March 26, 2003
Summary
The yeast protein Scp160p binds specific messenger RNAs (mRNAs), not random ones. Loss of Scp160p affects mRNA abundance and localization, showing its biological relevance in regulating gene expression.
Area of Science:
- Molecular Biology
- Yeast Genetics
- RNA Biology
Background:
- Scp160p is a yeast protein known to bind messenger RNA (mRNA) on polyribosomes.
- It remains unclear if Scp160p associates with specific or random mRNAs.
Purpose of the Study:
- To determine if Scp160p binds specific mRNAs.
- To investigate the biological relevance of Scp160p-mRNA interactions.
Main Methods:
- Affinity isolation of Scp160p-containing complexes.
- Microarray analysis of associated RNAs.
- Quantitative RT-PCR validation.
- Analysis of mRNA distribution upon Scp160p loss.
Main Results:
- Scp160p specifically associates with certain mRNAs, including DHH1, YOR338W, BIK1, YOL155C, and NAM8.
- Loss of Scp160p alters the abundance and localization of YOR338W mRNA.
- Scp160p depletion causes a shift in DHH1 and YOR338W mRNA from polyribosomes to mRNPs.
Conclusions:
- Scp160p is a specific RNA-binding protein in yeast.
- Scp160p plays a biologically relevant role in mRNA regulation and localization.