Related Experiment Videos
Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
Philip Hugenholtz1, Thomas Huber1
1ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia.
Abstract:
A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.