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Expression of Id2 mRNA in neuroblastoma and normal ganglion
Y Sato1, Y Kobayashi, H Sasaki
1Department of Surgery II, Nagoya City University Medical School, Japan.
Aims:
Inhibitors of DNA binding 2 (Id2) has been reported to be overexpressed in neuroblastoma cell lines carrying extra copies of the N-myc gene. It has been suggested that Id2 may be involved in the disturbed regulation of cell cycle by interfering with retinoblastoma protein.
Methods:
In this report we assessed Id2 gene expression in 20 neuroblastoma samples and eight normal ganglion tissues using quantitative reverse transcription-PCR (RT-PCR). Id2 expression in resected clinical samples of neuroblastoma needs to be studied.
Results:
Id2 messenger RNA (mRNA) was expressed in all the neuroblastoma samples. The level of Id2 mRNA expression was not influenced by the patient's age, gender, tumor's clinical stage, DNA ploidy pattern, histological pattern, the level of N-myc mRNA expression and whether the patient was found by mass screening or by symptom. Id2 was also expressed in comparable levels in normal differentiated sympathetic ganglion in adult.
Conclusion:
Our data suggest that Id2's role in the oncogenesis or progression of neuroblastoma is minimal.
Insights
Inhibitors of DNA binding 2 (Id2) expression was observed in all neuroblastoma samples. However, Id2 levels did not correlate with clinical factors, suggesting a minimal role in neuroblastoma oncogenesis.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Inhibitors of DNA binding 2 (Id2) overexpression is linked to neuroblastoma cell lines with extra N-myc gene copies.
- Id2 is hypothesized to disrupt cell cycle regulation by interfering with retinoblastoma protein.
Purpose of the Study:
- To assess Inhibitors of DNA binding 2 (Id2) gene expression in neuroblastoma patient samples.
- To investigate the correlation between Id2 expression and clinical parameters in neuroblastoma.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to measure Id2 mRNA levels.
- Id2 expression was analyzed in 20 neuroblastoma samples and 8 normal ganglion tissues.
Main Results:
- Id2 mRNA was detected in all analyzed neuroblastoma samples.
- Id2 expression levels were not influenced by patient age, gender, tumor stage, DNA ploidy, histology, N-myc expression, or detection method.
- Comparable Id2 expression was found in normal differentiated sympathetic ganglia.
Conclusions:
- The study suggests a minimal role for Inhibitors of DNA binding 2 (Id2) in the oncogenesis or progression of neuroblastoma.
- Id2 expression does not appear to be a significant prognostic marker in this cohort.