Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular imaging and cancer.

Ronald G Blasberg1

  • 1Department of Neurology and Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.

Molecular Cancer Therapeutics
|March 27, 2003
PubMed
Summary

Molecular imaging uses reporter genes and probes for noninvasive in vivo studies. This technology enables tracking gene expression and biological processes, with potential for clinical translation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased tumor glycolysis is associated with decreased immune infiltration across human solid tumors.

Frontiers in immunology·2022
Same author

Lactate Dehydrogenase A Depletion Alters MyC-CaP Tumor Metabolism, Microenvironment, and CAR T Cell Therapy.

Molecular therapy oncolytics·2020
Same author

The AQARA Principle: Proposing Standard Requirements for Radionuclide-Based Images in Medical Journals.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2020
Same author

<sup>18</sup>F-Fluorocholine PET uptake correlates with pathologic evidence of recurrent tumor after stereotactic radiosurgery for brain metastases.

European journal of nuclear medicine and molecular imaging·2019
Same author

Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2019
Same author

Inhibition of prostate cancer proliferation by Deferiprone.

NMR in biomedicine·2017

Area of Science:

  • Molecular biology
  • Cell biology
  • Imaging technology

Background:

  • Molecular imaging, a decade-old discipline, integrates molecular biology, cell biology, and imaging.
  • Noninvasive in vivo molecular imaging utilizes magnetic resonance, nuclear (PET, gamma camera), and optical systems.
  • Current strategies often employ indirect methods, coupling reporter genes with probes to infer gene expression levels.

Purpose of the Study:

  • To review indirect molecular imaging strategies for therapeutic transgenes.
  • To discuss examples of imaging endogenous biological processes in animals.
  • To address challenges in translating noninvasive molecular imaging to clinical applications.

Main Methods:

  • Utilizing reporter gene constructs with inducible or constitutive promoters.
  • Employing complementary reporter probes for signal detection.
  • Applying positron emission tomography (PET) imaging with radiolabeled probes.

Main Results:

  • Demonstrated indirect imaging of p53-dependent gene expression.
  • Showcased tracking of T-cell receptor-dependent activation and trafficking.
  • Reviewed the use of reporter constructs for monitoring biological processes.

Conclusions:

  • Indirect molecular imaging offers a powerful tool for studying gene expression and biological processes in vivo.
  • Reporter gene strategies provide insights into gene activity and cellular functions.
  • Clinical translation of noninvasive molecular imaging faces challenges that require further investigation.

Related Experiment Videos