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Open-tubular capillary electrochromatography using a polymeric surfactant coating.
Mary W Kamande1, Constantina P Kapnissi, Xiaofeng Zhu
1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Electrophoresis
|March 27, 2003
Summary
A novel polyelectrolyte multilayer (PEM) coating offers stable and reproducible stationary phases for open-tubular capillary electrochromatography (o-CEC). This method enhances separation selectivity for phenols and benzodiazepines with excellent endurance.
Area of Science:
- Analytical Chemistry
- Separation Science
- Materials Science
Background:
- Open-tubular capillary electrochromatography (o-CEC) requires robust stationary phases for efficient separations.
- Developing stable and selective coatings is crucial for advancing chromatographic techniques.
Purpose of the Study:
- To investigate a stable polyelectrolyte multilayer (PEM) coating for open-tubular capillary electrochromatography (o-CEC).
- To evaluate the selectivity, reproducibility, and endurance of the PEM coating as a dynamic stationary phase.
Main Methods:
- A simple coating procedure involving alternate rinsing of cationic poly(diallyldimethylammonium chloride) and anionic poly(sodium undecylenic sulfate) onto fused-silica capillaries.
- Electrochromatographic experiments were performed to assess separation performance.
- Reproducibility was evaluated by measuring electroosmotic flow (EOF) relative standard deviations (RSDs).
Main Results:
- The PEM coating demonstrated good selectivity for both phenols and benzodiazepines.
- Excellent reproducibility was achieved with run-to-run and capillary-to-capillary EOF RSDs below 1.5%.
- The coating exhibited high endurance, performing reliably for over 100 runs.
Conclusions:
- The developed PEM coating is a stable and effective dynamic stationary phase for o-CEC.
- This technique offers significant improvements in separation compared to bare capillaries.
- The PEM coating provides a viable alternative for benzodiazepine separation compared to micellar electrokinetic chromatography (MEKC).