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Transfection of articular chondrocytes with rhBMP7 gene and its expression
Deyu Duan1, Jingyuan Du, Yong Liu
1Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Abstract:
In order to investigate the possibility of expression of exogenous gene in transduced articular chondrocytes, plasmid pcDNA3-rhBMP7 was delivered to cultured chondrocytes. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. And the bioactivity of transgene expression product was detected through MTT assay as well. It was confirmed that exogenous gene could be expressed efficiently in transduced chondrocytes and the transgene expression product had obvious bioactivity. The present study provided a theoretical basis for gene therapy on the problems of articular cartilage.