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Lipid membrane with low proton permeability
Débora Nakai Biloti1, Maria Helena Andrade Santana, Francisco Benedito Teixeira Pessine
1Department of Physical Chemistry, Institute of Chemistry, State University of Campinas (UNICAMP), Cx Postal 6154, 13084-971 Campinas, SP, Brazil.
Abstract:
This work reports the production of a liposomal formulation, having a lipidic membrane with known chemical composition and a low proton permeability, as confirmed by physicochemical characterization of the maintenance of a transmembranic pH gradient. These liposomes consist of DSPC, DSPE-PEG, DSPG and cholesterol, with low internal pH. To verify the low proton permeability of these liposomal bilayers, a study of proton migration according to the fluorescence quenching of 9-aminoacridine (9AA), as well as CPT-11 encapsulation, were used to monitor the acidification of the intravesicular space. Both experiments showed that this liposomal formulation is able to maintain a transmembranic proton gradient.