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Characterization of rotaviruses causing diarrhoea in Vietnamese children

M E Landaeta1, W Dove, Ha Vinh

  • 1Department of Medical Microbiology, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.

Annals of Tropical Medicine and Parasitology
|March 29, 2003
PubMed

Insights

This study investigated viral causes of acute watery diarrhea in Vietnamese children, finding diverse rotavirus strains and novel variants. Understanding rotavirus evolution is crucial for developing effective vaccines.

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Acute watery diarrhea is a significant health concern in children, particularly in tropical regions.
  • Viral enteropathogens, especially rotavirus, are major causes of childhood diarrhea globally.
  • Limited data exists on the molecular epidemiology of rotaviruses in Vietnam.

Purpose of the Study:

  • To identify viral enteropathogens in children with acute watery diarrhea in Ho Chi Minh City, Vietnam.
  • To characterize the molecular diversity of detected rotaviruses, including G- and P-genotyping.
  • To investigate rotavirus evolution and inform vaccine development strategies.

Main Methods:

  • Faecal samples from 123 children were screened using negative-stain electron microscopy.
  • Rotavirus isolates underwent molecular analysis: electropherotyping, G- and P-genotyping via reverse-transcriptase PCR, and amplicon sequencing.
  • Novel PCR primers were utilized for enhanced genotyping.

Main Results:

  • Rotavirus was the predominant enteropathogen identified in 49 children.
  • Novel variants of G1 and P[8] genotypes were the most common, with significant sequence divergence from the Wa prototype.
  • Unusual G- and P-genotype combinations were observed, and 8% of children had co-infections with multiple rotaviruses.

Conclusions:

  • The study highlights the significant diversity and ongoing evolution of human rotaviruses in Vietnam.
  • Detection of novel variants and unusual genotype combinations underscores the need for continuous surveillance.
  • These findings provide valuable data for the rational design and implementation of rotavirus vaccines.

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