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[Effect of different storage methods on the viability of Halobacterium salinarum]
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
It has been established that the studied halobacteria are cryolabile and sensitive to the sharp change in osmotic pressure of the environment. Optimal temperature of their storage is +20 degrees C for suspension of native cells and +4 degrees C for the cells in anabiotic state. Their storage medium should have optimal osmotic pressure and contain NaCl and K+, Mg+ ions. It is recommended to keep halobacteria during one year in a form of suspension in nutrient medium, and during at least 4 years in lyophilized state. Other long-term storage methods: L-drying, freezing at low temperatures, are not recommended for halobacteria.