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[Screening for inhibitor of vascular endothelial growth factor from random peptide library]
Jie Wu1, Hongbin Zhang, Jie Wang
1Department of Medical Research, Guangzhou PLA General Hospital, Guangzhou 510010, China.
Objective:
To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.
Methods:
Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.
Results:
Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.
Conclusion:
The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.