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Optimization of a solid-phase extraction method for determination of indapamide in biological fluids using
Dragica Zendelovska1, Trajce Stafilov, Marina Stefova
1Institute of Preclinical and Clinical Pharmacology and Toxicology, Faculty of Medicine, Sts. Cyril and Methodius University, 50 Divizija bb, 1001 Skopje, Macedonia.
Summary
A new high-performance liquid chromatography (HPLC) method accurately quantifies indapamide in biological samples. This rapid assay simplifies indapamide determination in serum and whole blood using UV detection.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
- Biomedical Analysis
Background:
- Accurate quantification of indapamide in biological fluids is crucial for pharmacokinetic studies and therapeutic drug monitoring.
- Existing methods may lack the simplicity, speed, or sensitivity required for routine clinical or research applications.
Purpose of the Study:
- To develop and validate a simple, rapid, and sensitive high-performance liquid chromatographic (HPLC) method for determining indapamide concentrations in biological matrices.
- To establish a reliable analytical tool for indapamide analysis in serum and whole blood.
Main Methods:
- Solid-phase extraction (SPE) using RP select B cartridges for sample preparation.
- Reversed-phase HPLC on a C(8) column with a mobile phase of acetonitrile and buffered water.
- UV detection at 240 nm for indapamide quantification.
Main Results:
- The developed HPLC method demonstrated linearity over specified concentration ranges for serum (10.0-100.0 ng/ml) and whole blood (50.0-500.0 ng/ml).
- The limits of quantification were determined to be 10.0 ng/ml for serum and 50.0 ng/ml for whole blood.
- The method proved to be simple, rapid, and suitable for biological fluid analysis.
Conclusions:
- The developed HPLC-UV method provides a robust and efficient means for the accurate determination of indapamide in serum and whole blood.
- This assay facilitates pharmacokinetic investigations and clinical monitoring of indapamide therapy.
- The method's simplicity and speed make it a valuable tool in analytical and clinical laboratories.