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Related Experiment Videos

Characterization of crystalline drug nanoparticles using atomic force microscopy and complementary techniques.

Huaiqiu Galen Shi1, Leon Farber, James N Michaels

  • 1Pharmaceutical R&D, MRL. Merck & Co., Inc., West Point, Pennsylvania 19486, USA. galen_shi@merck.com

Pharmaceutical Research
|April 3, 2003
PubMed
Summary

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Atomic force microscopy (AFM) effectively images crystalline drug nanoparticles in liquid and solid forms, providing accurate size, shape, and distribution data. This technique offers superior visualization compared to light scattering for nanoparticle characterization.

Area of Science:

  • Pharmaceutical Sciences
  • Materials Science
  • Nanotechnology

Background:

  • Characterizing crystalline drug nanoparticles is crucial for formulation development.
  • Accurate determination of nanoparticle size, shape, and distribution is essential for predicting drug performance.
  • Existing methods may have limitations in providing comprehensive structural information.

Purpose of the Study:

  • To image crystalline drug nanoparticles in both liquid dispersion and solid dosage forms.
  • To determine the size, shape, and distribution of drug nanoparticles using advanced microscopy.
  • To evaluate the suitability of atomic force microscopy (AFM) for nanoparticle analysis.

Main Methods:

  • Adsorption of nanoparticles from a colloidal dispersion onto glass for AFM imaging.

Related Experiment Videos

  • Preparation of solid dosage forms by spray coating nanoparticles onto host beads.
  • Exposure of internal nanoparticles in solid dosage forms using ultramicrotomy for SEM and AFM examination.

    • Main Results:

    • AFM measurements indicated a mean nanoparticle diameter of 95 nm and an aspect ratio of 1.3.
    • Nanoparticles in the solid dosage form exhibited similar size and shape to those in the liquid dispersion.
    • AFM-derived particle size distribution correlated well with data from field emission scanning electron microscopy, static light scattering, and X-ray powder diffraction.

    Conclusions:

    • AFM is a valuable tool for visualizing and quantifying drug nanoparticle crystals within formulations.
    • AFM provides detailed shape and structural information not achievable with light scattering techniques.
    • Ultramicrotomy is an effective sample preparation method for examining the internal structure of solid dosage forms with minimal alteration.