Related Experiment Videos
Cloning, molecular characterization, and expression analysis of Copine 8
Rangan Maitra1, Dmitry N Grigoryev, Tapan Kumar Bera
1Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive Room 5120 MSC 4262, Bethesda, MD 20892-4264, USA.
Biochemical and Biophysical Research Communications
|April 3, 2003
Summary
Researchers identified and characterized Copine 8, a novel phospholipid-binding protein. This gene shows significant expression in the prostate, suggesting a key role in prostate regulation and development.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Copines are evolutionarily conserved, ubiquitous phospholipid-binding proteins.
- Understanding novel members of protein families is crucial for biological insight.
Purpose of the Study:
- To clone and perform molecular characterization of a new Copine family member, Copine 8.
- To investigate the expression patterns and potential functions of Copine 8.
Main Methods:
- Bioinformatic analysis of expressed sequence tags (ESTs) using the GoldenPath database.
- Gene cloning and molecular characterization.
- Analysis of gene expression across various tissues.
Main Results:
- Copine 8 was identified as a novel gene predominantly expressed in prostate and testis.
- Two distinct isoforms of Copine 8 were isolated.
- Copine 8 mRNA exhibited strongest expression in the prostate, heart, and brain, with lower levels in most other tissues.
Conclusions:
- Copine 8 is a newly characterized member of the Copine family.
- The expression profile of Copine 8 suggests a significant role in prostate regulation and development.