Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

3.5K
3.5K
Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges and opportunities in computational studies for lipid nanoparticle development.

npj drug discovery·2026
Same author

Antibiotics stimulate protein transfer to persister cells.

Science (New York, N.Y.)·2026
Same author

Ion-triggered reconfigurable hydrogels with salt-enhanced mechanical and swelling properties via network topological adaptation.

Nature communications·2026
Same author

Implantable living materials autonomously deliver therapeutics using contained engineered bacteria.

Science (New York, N.Y.)·2026
Same author

Rapid fabrication of solvent-compatible NOA 81 microfluidic devices for double-emulsion microfluidics.

Lab on a chip·2026
Same author

Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Reconfigurable Multistate Optical Memory in Mixed Halide Perovskites.

ACS applied materials & interfaces·2026
Same journal

Tunable, High-Relaxivity Gd(III)-Conjugated Lipoic Acid Hydrogels for Magnetic Resonance Imaging.

ACS applied materials & interfaces·2026
Same journal

Effects of Metal Ions of Metal-Organic Framework Membranes on the Transport of NaCl Solutions toward Seawater Desalination.

ACS applied materials & interfaces·2026
Same journal

Immobilization of Single Ni Sites and Separated Pd Clusters in Covalent Organic Framework for Enhanced Electrochemical Reduction of Nitrite to Ammonia.

ACS applied materials & interfaces·2026
Same journal

Evidence for Step-Edge-Assisted Large Hole Borophene on Ni(111).

ACS applied materials & interfaces·2026
Same journal

Growth Mode-Dependent Bi Incorporation and Carrier Localization in GaAsBi Wires.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

10.9K

Syringable Microcapsules for Sustained, Localized, and Controllable siRNA Delivery.

Yan Liu1, Yang Wang1, Rajesh A Kulkarni2

  • 1School of Engineering and Applied Sciences (SEAS), Harvard University, Cambridge, Massachusetts 02138, United States.

ACS Applied Materials & Interfaces
|December 20, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces microcapsules for sustained local delivery of small interfering RNA (siRNA), reducing injection frequency and toxicity. These microcapsules ensure constant siRNA release for 3 months, improving therapeutic durability.

Keywords:
PLGAbiodegradablecontrolled deliverymicrofluidicssmall interfering RNA (siRNA)syringable microcapsules

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.4K
Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

10.6K

Related Experiment Videos

Last Updated: Jun 4, 2025

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

10.9K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.4K
Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

10.6K

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • RNA Therapeutics

Background:

  • Current small interfering RNA (siRNA) and antisense oligonucleotide therapies often require invasive administration and frequent injections.
  • Challenges with existing formulations include side effects from bolus injections, high acute toxicity, and rapid clearance.
  • Need for improved delivery methods to enhance therapeutic durability and reduce patient burden.

Purpose of the Study:

  • To develop and evaluate a microcapsule-based system for sustained local delivery of cholesterol-conjugated siRNA.
  • To assess the encapsulation efficiency, loading capacity, syringeability, and in vitro/in vivo release profiles of the microcapsules.

Main Methods:

  • Fabrication of poly(lactic-co-glycolic acid) (PLGA) microcapsules using microfluidics with controlled size and shell thickness.
  • Evaluation of encapsulation efficiency, loading capacity, and syringeability (27-32 G needles) of the microcapsules.
  • In vitro release testing over 3 months, followed by subcutaneous injection in mice to determine in vivo release profiles.

Main Results:

  • Microcapsules achieved nearly 100% encapsulation efficiency and a high loading capacity (8900 μg siRNA/mg polymer).
  • 40 μm microcapsules demonstrated excellent syringeability, suitable for various injection routes.
  • In vitro and in vivo studies confirmed sustained siRNA release over 3 months, contrasting with rapid clearance of unencapsulated siRNA.

Conclusions:

  • PLGA microcapsules provide an effective platform for sustained local delivery of siRNA.
  • This method significantly extends siRNA therapeutic activity and reduces the need for repeated invasive administrations.
  • The developed microcapsules offer a promising approach to improve the safety and efficacy of RNA-based therapeutics.