Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A Crh-specific function in carbon catabolite repression in Bacillus subtilis.

Jessica B Warner1, Juke S Lolkema

  • 1Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Biological Center, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.

FEMS Microbiology Letters
|April 3, 2003
PubMed
Summary

The study reveals a specific role for the Crh protein in Bacillus subtilis gene regulation, distinct from the HPr protein, particularly when growing on non-carbohydrate sources like succinate and glutamate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Models to determine the kinetic mechanisms of ion-coupled transporters.

The Journal of general physiology·2019
Same author

Arginine and Citrulline Catabolic Pathways Encoded by the <i>arc</i> Gene Cluster of Lactobacillus brevis ATCC 367.

Journal of bacteriology·2018
Same author

Convergent evolution of the arginine deiminase pathway: the ArcD and ArcE arginine/ornithine exchangers.

MicrobiologyOpen·2016
Same author

Structure and elevator mechanism of the Na<sup>+</sup>-citrate transporter CitS.

Current opinion in structural biology·2016
Same author

ArcD1 and ArcD2 Arginine/Ornithine Exchangers Encoded in the Arginine Deiminase Pathway Gene Cluster of Lactococcus lactis.

Journal of bacteriology·2015
Same author

The Hill analysis and co-ion-driven transporter kinetics.

The Journal of general physiology·2015

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Carbon catabolite repression in Bacillus subtilis involves the HPr protein.
  • The related protein Crh has an unknown specific function.
  • Both HPr and Crh are involved in the phosphoenolpyruvate:carbohydrate phosphotransferase system.

Purpose of the Study:

  • Investigate the roles of HPr and Crh in repressing the citM gene.
  • Determine if Crh has a specific function in gene regulation.
  • Understand gene regulation during growth on different carbon sources.

Main Methods:

  • Studied Bacillus subtilis strains with mutations in HPr and Crh.
  • Grew bacteria in minimal media with glucose or succinate/glutamate.
  • Analyzed the repression of the citM gene encoding the Mg(2+)-citrate transporter.

Related Experiment Videos

Main Results:

  • Both HPr and Crh mediated full repression of citM on glucose.
  • Repression of citM on succinate and glutamate was specifically mediated by Crh.
  • Crh-deficient strains showed relieved repression on succinate/glutamate, unlike HPr mutants.

Conclusions:

  • This study provides the first evidence for a Crh-specific function in Bacillus subtilis.
  • Crh plays a distinct regulatory role, particularly during growth on non-carbohydrate substrates.
  • HPr and Crh have differential roles in carbon catabolite repression depending on the carbon source.