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Related Experiment Videos

Insulin containing polyethylenimine-dextran sulfate nanoparticles.

Waree Tiyaboonchai1, James Woiszwillo, Robert C Sims

  • 1Department of Pharmaceutical Chemistry, School of Pharmacy, The University of Kansas, 2095 Constant Ave., Lawrence, KS 66047-3729, USA.

International Journal of Pharmaceutics
|April 4, 2003
PubMed
Summary

A novel nanoparticle system using polyethylenimine (PEI) and dextran sulfate (DS) with zinc effectively encapsulates insulin. This biocompatible delivery system preserves drug activity and shows potential for prolonged hypoglycemic effects in diabetic rats.

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Mucoadhesive Silk Fibroin Nanoparticles for Drug Delivery to the Ocular Surface.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2025

Area of Science:

  • Polymer science
  • Nanotechnology
  • Drug delivery systems

Background:

  • Developing effective and stable nanoparticle delivery systems is crucial for therapeutic applications.
  • Controlling particle size and maintaining drug integrity are key challenges in nanoparticle formulation.

Purpose of the Study:

  • To develop an aqueous nanoparticle delivery system using oppositely charged polymers and zinc.
  • To optimize formulation parameters for particle size control and high drug entrapment.
  • To evaluate the stability and biological activity of encapsulated insulin.

Main Methods:

  • Utilized polyethylenimine (PEI) and dextran sulfate (DS) with zinc as a stabilizer.
  • Investigated the effects of pH, polymer weight ratio, and zinc sulfate concentration on particle size.

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  • Assessed drug entrapment efficiency, in vitro release, and conformational stability using circular dichroism (CD).
  • Evaluated biological activity in a diabetic rat model.

    • Main Results:

    • Achieved spherical nanoparticles (250 nm mean diameter) with a zeta potential of +30 mV under optimal conditions.
    • Demonstrated up to 90% insulin entrapment efficiency with no detected degradation.
    • Confirmed preservation of insulin's secondary structure and biological activity.
    • Observed rapid in vitro release but a prolonged hypoglycemic effect in vivo.

    Conclusions:

    • The developed aqueous nanoparticle system offers facile manufacturing with biocompatible polymers.
    • The system allows for controlled particle size, high drug entrapment, and preservation of protein structure and activity.
    • This formulation shows promise for sustained insulin delivery and managing diabetes.