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[Expression of basic fibroblast growth factor and fibronectin in tissue engineering skin allograft during healing
Junlin Wang1, Yan Jin, Zheng Guo
1Department of Pathology, Stomatological College, Fourth Military Medical University, Xi'an 710032, China.
Objective:
The aim of this study was to explore the expression of basic fibroblast growth factor (bFGF) and fibronectin during the healing process of allograft tissue engineering skin.
Methods:
The tissue engineering skin that was obtained from neonatal SD rats was cultured in the lab. Afterwards, the skin was grafted into adult SD rats, and the expressions of bFGF and fibronectin were detected on the 7th, 10th, 14th, 20th and 30th day after the allograft of the tissue engineering skin. The autografted skin in 15 adult Wistar rats and the normal skin in 15 Wistar were treated as the control. HE staining and immunohistochemical staining were used to examine the healing of grafted skin.
Results:
The expression of bFGF and fibronectin was the strongest on the 10th day after graft, and was weaker before the 10th day and after the 14th day. The expression changes of bFGF and fibronectin were similar as they were in the autograft group.
Conclusion:
The expression changes of bFGF and fibronectin in the tissue engineering skin during the process of wound healing were similar to those of autografted skin, and these can promote the repair of tissue engineering skin allograft.