Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelial-directed hepatic regeneration after partial hepatectomy.

Arin K Greene1, Stephen Wiener, Mark Puder

  • 1Department of Surgery, Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Hunnewell 103, Boston, MA 02115, USA.

Annals of Surgery
|April 5, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Angioedema Presenting as Infant-Onset Primary Lymphedema.

Journal of vascular anomalies·2026
Same author

Primary Upper Extremity Lymphedema Caused by a <i>CELSR1</i> Variant.

Journal of vascular anomalies·2026
Same author

Correction: MRC1 and LYVE1 expressing macrophages in vascular beds of GNAQ p.R183Q driven capillary malformations in Sturge Weber syndrome.

Acta neuropathologica communications·2026
Same author

Prenatal Lymphedema: A Genotype-Phenotype Analysis.

Journal of vascular anomalies·2026
Same author

Childhood-Onset Desmin-Related Cardiomyopathy: Comprehensive Clinical, Histopathological, Immunohistochemical, Ultrastructural, and Molecular Characterization.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2026
Same author

Rheumatological Diseases and Lower Extremity Edema: A Lymphoscintigram Study.

Journal of vascular anomalies·2026

The microvascular endothelium regulates liver regeneration after partial hepatectomy. Angiogenesis modulators like bFGF and TNP-470 impact endothelial cell proliferation and apoptosis, influencing liver mass recovery.

Area of Science:

  • Regenerative Medicine
  • Vascular Biology
  • Hepatology

Background:

  • Liver regeneration is crucial for restoring function after injury but the underlying mechanisms are not fully understood.
  • The microvascular endothelium's role in regulating organ mass, similar to its role in tumor growth, was hypothesized.
  • The liver's capacity to regenerate to its normal mass within eight days post-excision presents a model for studying organ regeneration.

Purpose of the Study:

  • To investigate the role of the microvascular endothelium in controlling regenerating liver mass following partial hepatectomy.
  • To determine if modulating angiogenesis affects liver regeneration dynamics.

Main Methods:

  • Partial hepatectomy was performed on mice, followed by administration of basic fibroblast growth factor (bFGF) to stimulate angiogenesis or TNP-470 to inhibit it.

Related Experiment Videos

  • Liver mass was measured at multiple time points post-surgery.
  • Immunohistochemistry was used to analyze endothelial cell proliferation and apoptosis.

    • Main Results:

    • Basic fibroblast growth factor (bFGF) significantly accelerated liver regeneration, increasing endothelial cell proliferation and decreasing apoptosis.
    • TNP-470 markedly inhibited liver regeneration by reducing endothelial cell proliferation.
    • Cessation of regeneration correlated with decreased endothelial proliferation and increased apoptosis.

    Conclusions:

    • Systemic administration of angiogenesis agents directly impacts liver mass regeneration by altering endothelial cell proliferation and apoptosis.
    • Endothelial cell apoptosis is linked to the termination of the regenerative process in control livers.
    • These findings identify the endothelial cell as a key regulator of adult tissue mass regeneration in the context of partial hepatectomy.