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Updated: Aug 15, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
Published on: August 27, 2015
Individual fatty acids influence cultured human liver cells differently when compared to plant oils
Bruce C Davidson1, Luis A F Girao, Alfredo Giangregorio
1University of the Witwatersrand Medical School, 7 York Road, Parktown, 2193, South Africa.
Abstract:
Most work to date using fatty acids and cultured cells has concentrated on single fatty acids, yet cells in vivo are exposed to fatty acid combinations in the form of triacylglycerols and phosphoglycerides. We have quantitated the influence of the individual fatty acids that make up three plant oils, as well as the plant oils themselves, on the growth of normal and transformed human liver cell lines. The effects of the individual fatty acids were then combined in proportion to their concentration in each plant oil and compared to the effects of the intact oils. The oils initially induced synergistic effects with low concentrations, but with high concentrations competitive effects were shown, when compared to the combined effects of the individual fatty acids.
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