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The pathogenesis and pathology of experimental Quaranfil virus infection
Abstract:
Mice were infected intranasally with Quaranfil arbovirus, and killed at intervals from 1 day to 2 months later. The infection produced clinical signs of neurological disturbance and a highly mortality. The virus could be isolated from the lungs on Days 1-9 and from the brain on Days 1-11 of the infection. Meningoencephalitis developed by Day 5 in the olfactory lobes and spread progressively caudally, involving all regions of the brain by Day 7. The prinicipal features of the inflammatory process were perivascular cuffing, necrosis of neurones, and, in the later stages, spongiform degeneration and marked astrocytic and microglial activity. In the lungs after a short and mild exudative phase interstitial pneumonia developed. This was characterized by proliferation of connective tissue cells in interalveolar septa and later by fibrosis.
Insights
Quaranfil arbovirus infection in mice caused severe neurological disturbances and high mortality. The virus targeted the brain and lungs, leading to meningoencephalitis and interstitial pneumonia with fibrosis.
Area of Science:
- Virology
- Neuropathology
- Immunology
Background:
- Quaranfil arbovirus is an arbovirus with potential pathogenic effects.
- Understanding its impact on host physiology is crucial for disease management.
Purpose of the Study:
- To investigate the pathological effects of Quaranfil arbovirus infection in a mouse model.
- To characterize the temporal progression of viral dissemination and associated neuropathology and lung pathology.
Main Methods:
- Intranasal inoculation of mice with Quaranfil arbovirus.
- Serial necropsies and sample collection at various time points (1 day to 2 months).
- Histopathological examination of brain and lung tissues to assess inflammatory and degenerative changes.
Main Results:
- Clinical signs of neurological disturbance and high mortality were observed.
- Viral isolation confirmed presence in lungs (Days 1-9) and brain (Days 1-11).
- Progressive meningoencephalitis, neuronal necrosis, spongiform degeneration, and glial activation were noted in the brain. Interstitial pneumonia with fibrosis developed in the lungs.
Conclusions:
- Quaranfil arbovirus infection leads to severe meningoencephalitis and interstitial pneumonia in mice.
- The study elucidates the neuropathological and pulmonary sequelae of this arbovirus infection.