Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in chromosomal surface structure by different isolation conditions.

Takefumi Sone1, Megumi Iwano, Shouhei Kobayashi

  • 1Department of Biotechnology, Osaka University, Graduate School of Engineering, Suita, Osaka, Japan.

Archives of Histology and Cytology
|April 12, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rectal Cancer Surgery in Nonagenarians: A Multi-Institutional Study of Feasibility and Risk-Stratified Outcomes.

World journal of surgery·2026
Same author

NOTE Alterations in Gene Expression Profiles of Human Epidermal Cells Treated with Jojoba Oil.

Journal of oleo science·2026
Same author

Indispensable roles of RBOH-mediated ROS production in development and morphogenesis of Marchantia polymorpha.

Current biology : CB·2026
Same author

A SABATH family enzyme regulates development in part via the gibberellin-related pathway in the liverwort Marchantia polymorpha.

Plant & cell physiology·2026
Same author

[A Case of Gastric Cancer Diagnosed from Adrenal Metastasis after Left Adrenal Tumor Resection and Treated with Robot-Assisted Distal Gastrectomy after Chemotherapy].

Gan to kagaku ryoho. Cancer & chemotherapy·2026
Same author

Surgical outcomes and patient selection in nonagenarians with colon cancer: a comparative multi-institutional study of laparoscopic and open approaches.

Langenbeck's archives of surgery·2025

Human metaphase chromosomes isolated using two methods show different surface structures. This difference is linked to the removal of linker histones (H1) in one method, affecting chromatin fiber appearance.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Chromatin Structure

Background:

  • Human metaphase chromosomes exhibit diverse surface structures under scanning electron microscopy (SEM).
  • Previous SEM studies have reported varying chromosome morphologies, leading to potential confusion in interpretation.

Purpose of the Study:

  • To investigate the reasons behind the differing SEM appearances of human metaphase chromosomes.
  • To determine the role of isolation buffers and associated protein content in observed morphological variations.

Main Methods:

  • Synchronization of the human cell cycle.
  • Isolation of metaphase chromosomes using centrifugation with two distinct solutions: polyamine buffer (PAB) and citric acid solution (CAS).
  • Analysis of isolated chromosomes by scanning electron microscopy (SEM) and protein extraction.

Related Experiment Videos

Main Results:

  • Sufficient yields of human metaphase chromosomes were obtained by centrifugation.
  • SEM revealed two distinct surface structures: PAB-isolated chromosomes were smooth with scaly structures, while CAS-isolated chromosomes showed a dense fibrous surface (50-70 nm diameter).
  • Protein analysis indicated the removal of linker histones (H1) from CAS-isolated chromosomes.

Conclusions:

  • The observed differences in human chromosome SEM images are attributed to variations in isolation methods.
  • Removal or depletion of linker histones (H1) influences the surface structure and prevents scattering of chromatin fibers.
  • This study clarifies the morphological variability of chromosomes observed via SEM.