Related Experiment Video
Updated: Sep 26, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
The binding potential between the cholera toxin B-oligomer and its receptor
1Physics Department, University of Vermont, Cook Building, Burlington, Vermont 05405, USA.
Abstract:
Employing atomic force microscopy as an in situ molecular force probe, we have measured the binding strength between cholera toxin B-pentamer (ctB) and its membrane receptor, ganglioside G(M1). By application of the basic principle of the reaction rate theory, key parameters of the ligand-receptor binding potential can be obtained from our data. The potential has a well with a spatial span of about 2.5 A and a depth of at least six times the thermal energy at room temperature. The very short range nature of the binding potential leads to the specificity of the ctB-G(M1) coupling.
More Related Videos
09:39Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
Published on: May 30, 2013
07:58Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Related Concept Videos
Cholera
The Two-State Receptor Model
The binding affinity of a drug determines its interaction with one...
The Equilibrium Binding Constant and Binding Strength
Bacterial Toxins
GPCRs Regulate Adenylyl Cylase Activity
Two...
Ligand Binding Sites
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...